This study examined the intensities of 1,600m run/walk indicated by the heart rate in college students, and the stress variability corresponding to the intensities. Thirty-one males (21.16±1.37 yrs, 176.30±5.15 cm, 71.68±8.73kg) and 32 females (20.97±1.30 yrs, 164.34±4.49 cm, 57.31±4.88kg) of college students were the subjects of this study. Heart rates were recorded by the Polar heart rate monitor (PST 4000), and then converted into intensities (%HRR) via the Karvonen method. The data were analyzed by descriptive statistics and dependent t-test (α=.05). The results indicated that the intensities of 1600m run/walk were about "high-middle intensity". The 1,600m run/walk test might not only serve as an effective measure for cardiorespiratory endurance but also as an enhancement for the aerobic power on college students. Besides, when performing 1,600m run/walk, the heart rates turned to be in a steady state for 1 minute just before the stage of intensity climbed up to "high intensity". Based on the results, suggestions were made as to the 1,600m run/walk for both the examiners and the runners.