This paper examined the influence of three different exercise prescriptions on physiological index and humoral immunity of weight-reduced overweight persons. The subjects consisted of 21 female university students whose BMIs (Body Mass Indexes) were larger than 25kg/m2. They were randomly divided into three groups; each group had seven subjects. Their diet would not be changed, but they were assigned to three different exercise prescriptions groups. All exercise prescriptions included an aerobic exercise (walking) session for a six-week duration and five times per week. The three groups were different in terms of exercise intensity (GroupⅠ: 50%-55% HRmax; Group Ⅱ: 60%-65% HRmax ; and GroupⅢ: 70%-75% HRmax and exercise duration (GroupⅠ: 60 minutes, Group Ⅱ: 50 minutes, GroupⅢ: 40 minutes). The anthropometric data (weight, height, body fat percentage and circumference of waist and hip) and physiological indexes (immunoglobulin IgA, IgG and IgM) were measured 48 hours before and after the exercise session. After the six-week aerobic exercise, all subjects in the three groups significantly improved (p<.05) in weight, BMI and body fat percentages. Although all subjects improved in the waist and hip ratios, only subjects in group Ⅲ reached the significant level (p<.05). Regarding the immunoglobulin response, both GroupⅠand Group Ⅱ significantly increased (p<.05) in IgA, IgG and IgM. Besides, IgG and IgM did not change in Group Ⅲ, while significantly decreased in IgA (p<.05). The exercise prescriptions for GroupsⅠand Ⅱ could improve the anthropometric indexes as well as the immune response. Thus, these two exercise prescriptions could be served as safe and feasible methods for weight reduction and enhancement of the immune functions.