Up to the present, there have been few documents providing the actual measuring methods that could calculate the kinematics parameters (KPs) of the free rotational ball. Most studies just mentioned the KPs' influence on aerodynamic lift force, but there was no valid and actual measuring method for research. This study was to develop an effective method for measuring the spin rate and the axis of the free rotational ball, and estimate the accuracy of this method. The variation of the simulated spherical surface that rotated in the sky was taken and the mathematical principle of perpendicular bisecting plane in measuring the spin axis of the three-dimensional rotational ball was applied. The study employed 2 JVC digital cameras with 120 Hz in the course of shooting. Furthermore, the films were digitized with APAS software (Ariel Performance Analysis System) to determine the instantaneous axis of rotation and the spin rate of the ball. The results of the study showed that this study utilized 3-D cinematography analysis and connected with the mathematical principle of perpendicular bisecting plane to develop a kind of method to effectively and actually measure the spin rate and the axis of the free rotational ball in the sky by the displacement changes of any two markers that marked the point on the surface of the sphere. The overall accuracy for SEE% was 5.2% and 1.8% respectively. This study provided the important rotation measurement bases for some relevant studies such as aerodynamics.