The purpose of this study was to investigate the differences between the health-related physical fitness and trend analysis of five-year college male students in Tung-Fung Institute of Technology. The subjects of this study were one hundred and forty-nine males had accepted the health-related fitness test by the Ministry of Education, Taiwan, R.O.C. from 88 to 92 academic years. The findings are results as follows : 1. The third grade had the best performance on Sit-reach, Broad jump, Sit-ups/one min and 1600 m run and walk then was the second grade and forth grade students with quadratic trend presented. 2. On BMI, there was linear trend presented, and increased year-by-year with age-increasing; so the third grade had the best performance on physical fitness then was the second grade, except for BMI, the other physical fitness physical fitness test were quadratic trend presented.