This paper aims to investigate the factors impacting training and knowledge transfer for nurses in Taiwan. Applying questionnaires survey, 336 nurses from 16 hospitals were sampled as a unit of analysis to test the influence of personal characteristics of nurses on both nurses' training experiences and their knowledge transfer behavior at Taiwanese hospitals. The results shown in t-test and ANOVA statistics suggest that nurses' characteristics have significant impacts on training and knowledge transfer. Moreover, this survey also showed that the influence of age, education level, and work experiences on training experience and knowledge transfer is significant. Nurses whose age is higher have higher levels of agreement with "Training Frequency" and "Sharing". Likewise, nurses whose employment is longer and education higher have higher levels of agreement with "Training Frequency", "Willing", "Ability" and "Sharing". Accordingly, to provide the experience and knowledge for young and new nurses, hospital management can hold more training programs to enhance their ability. In doing so, the main purpose of this study through submitting a knowledge cycle to verify the function of training of nurse as the facilitator of new knowledge transfer in practice can be achieved. Hospital services should always support a philosophy that nurses who obtained "good training" of knowledge transfer at their work field are more likely to enhance their own "new knowledge".