The purpose of this study was to investigate the correlation and the difference of maximal oxygen uptake among running on treadmill and 1600m running and 12 minutes running for the collegiate male students. There were 24 healthy collegiate male students were selected as the subjects of this study, and mean age was 20.4±1.7 yrs., mean height was 173.4±5.3 cm, and mean weight was 67.7±7.2 kg. Each subject was asked to receive the test of maximal oxygen uptake and the test of 1600 meters run and the test of 12 minutes run alternately. After data treatment and analysis, the mean maximal oxygen uptake running on treadmill obtained 50.78±4.84ml/kg/min, and the mean maximal oxygen uptake of 1600m run received 50.36±5.32ml/kg/min, and the mean maximal oxygen uptake of 12 minutes run showed 0±4.88 ml/kg/min. After running the repeated ANOVA statistic analysis, the results showed there were no significant difference among the three kinds of running test(p>.05). There was a high correlation of maximal oxygen uptake between the test of running of treadmill and 1600m run, and there was also a medium correlation of maximal oxygen uptake between the test of running on treadmill and the test of 12 minutes run. From the results of high correlation of maximal oxygen uptake between the test on treadmill run and the test of 1600m run, the test of 1600 meters run showed high economics and high efficiency benefit for test the crowd people for the cardio-fitness.