高密度脂蛋白被美國國家膽固醇教育小組(National Cholesterol Education Program)認定為優質膽固醇，因其能吸收沈澱於血管璧的低密度脂蛋白而轉送至肝臟形成能量轉化。最近研究動脈硬化及冠狀心臟疾病病變的學者，都認定膽固醇對高密度脂蛋白的比例較為重要，其比例愈少危險愈小，因此近年來其膽固醇／高密度脂蛋白比例成為認定是心臟病變最有用的指標。目的：探討電動跑步機衰竭運動後2 小時在膽固醇/高密度脂蛋白比例的差異。方法：本研究以12位健康男性為受試者，年齡平均為22.59±3.31歲，身高為170±5.48公分，體重為62.5±4.65公斤，在受試前接受12~14小時食物控制，而後參加電動跑步機衰竭運動，運動前及運動後2小時接受血液檢驗，電動跑步機依Durstine等的方法操作（1.4 mph 速度及0%坡度為準備運動，4.4 mph速度及4%坡度為主要運動），膽固醇用 CHOD-PAP法檢測，高密度脂蛋白用Phosphotungastic acid-mgcl2 Precipitant 法檢測，t-test 用於比較差異性。結果：從t-test 檢定，證實電動跑步機衰竭運動前後，在膽固醇對高密度脂蛋白的比例不具差異性(3.53/3.52)，膽固醇在衰竭運動後略為升高4.82%(172.7-181 mg/dl)，高密度脂蛋白在電動跑步機運動後升高10.37% (50.6-55.83mg/dl)。結論：從資料分析與實驗證實長時間的本次衰竭運動對膽固醇／高密度脂蛋白的比例沒有影響。

High density lipoprotein cholesterol (HDL-C) has been identified a good cholesterol by National Cholesterol Education Program. Since HDL-C pick up cholesterol from peripheral tissue and transport it to the liver to protect atherosclerotic. Many studies have shown that atherosclerotic and heart disease are not only the level of total cholesterol (TC), low density lipoprotein (LDL-C) and HDL-C. The ratio of TC to HDL-C has been extremely useful in estimating heart disease risks (CHD). Purpose: To detect the ratio of TC to HDL-C in treadmill exhaustive exercise. Method: In the experiment, 12 male subjects were referred for samples. Their venous blood were examined before and after 2 hrs of exercise. The subjects age are 22.59±3.31 yrs, body height are 170±5.48 cm and body weight are 62.5±4.63 kg respectively. All samples received 12~14 hrs diet control before exhaustive exercise. Durstine's treadmill exercise protocol are used in the experiment. TC was measured by CHOD-PAP method and HDL-C was quanlified by phosphotungastic acid-mgcl2 precipitant method. T-test was used to identify the significant level. Result: From t-test, the ratio of TC to HDL-C after 2 hours of exhaustive treadmill exercises is not different significantly. TC increase 4.83%, HDL-C increase 10.37% and the ratio of TC/HDL-C is decreased from 3.53 to 3.52. Conclusion: From data analysis that the ratio of TC to HDL-C is not different in this treadmill exercise.