The purposes of this study were to evaluate the effectiveness of teaching and learning of biodiversity on elementary school 5(superscript th) graders. A quasi-experimental design was used with 4 classes. Two classes were assigned as the experimental group, and the others were the control group. Students in experimental group were given 6 weeks of biodiversity teaching activities, while the control group received traditional instruction. Two research instruments were used in this study include biodiversity achievements test and environmental attitude and behavior scales. The results were undergone by ANCOVA analysis. The major findings of this study were summarized as following: 1. The biodiversity concepts of experimental group students were significant improved than the control group did. 2. The environmental attitude and behavior of experimental group students were positively improved than the control group did. 3. This study strongly suggests that the biodiversity issues and learning activities could be integrated into elementary school level.