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題名:常見織物上微量生物跡證之採樣效能評估
書刊名:刑事科學
作者:呂政鴻柳國蘭粘凱俐吳昆典陳振銘程曉桂侯劭毅
作者(外文):Lu, Cheng-hungLiu, Kuo-lanNien, Kai-liWu, Kun-tienChen, Chen-mingCheng, Hsiao-kueiHou, Shao-yi
出版日期:2011
卷期:70
頁次:頁113-123
主題關鍵詞:微量DNADNA萃取Minitape
原始連結:連回原系統網址new window
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  • 點閱點閱:70
DNA鑑識過程的第一步是生物跡證採集,特別是對於微量細胞的跡證例如手套、汗漬斑跡等,若是無法蒐集到足夠量的細胞,常常無法成功檢出DNA型別而失去破案先機,如何提升採集效能是非常重要的課題。本研究以同型式之棉布手套給5位不同受測者穿戴並從事不同勞務2小時,再放置至少1天後進行DNA檢測,每位受測者提供3份手套樣本,其中第一、二份手套採樣食指第一指節指腹及虎口處1.5cm×1.5 cm範圍處,第一份手套以剪取方式採樣,第二份手套以Scenesafe FASTTM微量證物膠帶(DNA free;ETO sterilised)黏取方式採樣,第三份手套以Scenesafe FASTTM膠帶大範圍黏取手套內側所有部份(包含5根手指指腹處、虎口、掌心等處),至膠帶沒有黏性為止,實驗結果顯示以Scenesafe FAST TM大範圍黏取之採樣效果較佳,所有(5位)受測者均可成功檢出DNA型別;在手套特定位置以剪取或黏取方式進行採樣及DNA鑑定,從定量結果顯示以剪取方式所獲得的DNA量較多,但兩者之DNA型別檢出比率並無顯著差異。 另外為評估不同材質織物上之微量斑跡以四種不同方式進行採樣,其對DNA檢出結果之影響,利用已知量之人類角質化細胞株HaCaT,等量滴於棉質、尼龍、牛仔布等三種不同材質織物上,陰乾三日後,分別以剪取、黏取、Double swab(wet/dry)等四種不同方式進行採樣及DNA鑑定,從定量結果顯示直接剪取效果優於其他方式,但牛仔布以直接剪取方式採樣結果發現DNA定量有抑制情形,Scenesafe FASTTM膠帶黏取之效果優於Double swab方式。本研究評估以膠帶採樣棉質手套跡證之可行性與優缺點,同時比較不同材質織物之最佳DNA跡證採樣方法,可供他實驗室參考,進而提升鑑定水平。
期刊論文
1.Raymond, J. J.、van Oorschot, R. A. H.、Walsh, S. J.、Roux, C.(2008)。Trace DNA analysis: Do you know what your neighbor is doing? A multi-jurisdictional survey。Forensic Science International: Genetics,2,19-28。  new window
2.Budowle, B.、Baechtel, F. S.、Comey, C. T.、Giusti, A. M.、Kievan, L.(1995)。Simple protocols for typing forensic biological evidence: Chemiluminescent detection for human DNA quantitation and restriction fragment length polymorphism (RELP) analyses and manual typing of polymerase chain reaction (PCR) amplified polymorphisms。Electrophoresis,16,1559-1567。  new window
3.Wickenheiser, R. A.(2002)。Trace DNA: a review, discussion of theory, and application of the transfer of trace quantities of DNA through skin contact。Journal of forensic sciences,47,442-450。  new window
4.Pang, B.、Cheung, B.(2007)。Double swab technique for collecting touched evidence。Legal Medicine,9,181-184。  new window
5.Larkin, A.、Harbison, S. A.(1999)。An improved method for STR analysis of bloodstained denim。International Journal of Legal Medicine,112,388-390。  new window
6.Shaw, K.、Sesardi, I.、Bristol, N.、Ames, C.、Dagnall, K.、Ellis, C.、Whittaker, F.、Daniel, B.(2008)。Comparison of the effects of sterilisation techniques on subsequent DNA profiling。International Journal of Legal Medicine,122,29-33。  new window
7.Deragon, J. M.、Sinnett, D.、Mitchell, G.、Potier, M.、Labuda, D.(1990)。Use of gamma irradiation to eliminate DNA contamination for PCR。Nucleic acids research,18,6149。  new window
8.Hansson, O.、Finnebraaten, M.、Heitmann, I. K.、Ramse, M.、Bouzga, M.(2009)。Trace DNA collection- Performance of minitape and three different swabs。Forensic Science International: Genetics Supplement Series,2,189-190。  new window
9.Benschop, C. C. G.、Wiebosch, D. C.、Kloosterman, A. D.、Sijen, T.(2010)。Post-coital vaginal sampling with nylon flocked swabs improves DNA typing。Forensic Science International: Genetics,4,115-121。  new window
10.Phipps, M.、Petricevic, S.(2007)。The tendency of individuals to transfer DNA to handled items。Forensic science international,168(2/3),162-168。  new window
11.Alessandrini, F.、Cecati, M.、Pesaresi, M.、Turchi, C.、Carle, F. A.(2003)。Tagliabracci, Fingerprints as evidence for a genetic profile: morphological study on fingerprints and analysis of exogenous and individual factors affecting DNA typing。Journal of forensic sciences,48,586-592。  new window
12.Linacre, A.、Pekarek, V.、Swaran, Y. C.、Tobe, S. S.(2010)。Generation of DNA profiles from fabrics without DNA extraction。Forensic Science International: Genetics,4,137-141。  new window
會議論文
1.陳珮瑜、劉佳玲、柳國蘭、程曉桂(2009)。膠帶黏膠面指紋顯現方法對DNA萃取、STR型別分析之影響。鑑識研討會,331-336。  延伸查詢new window
 
 
 
 
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