In this study, we use the moisture transferring and the cooling function of the fabric in the female version T-shirt on the research on human comfort, and compared with the cool stone ore composite clothing and only the clothing has a moisture transferring, these two clothing analysis, with the infrared thermal imaging analysis of the gap between heart rate variability and heart rate variability analysis by instrument in sympathetic activity and parasympathetic activity of numerical values to calculate the Cool wicking fabric and fabric in the dynamic and static difference in comfort.