To compare various physiological indexes during exercise tests between Amis and non-Amis (nAmis) college baseball players. Ten Amis and 10 nAmis healthy college baseball players participated in this study. Treadmill and indirect calorimetry were used to assess physiological indexes of fitness during three different exercise tests. Finger-tip blood samples were collected during rest, right before and after exercise, and then analyzed. Amis group had significantly lower VO2max (Amis: 48.12 ± 5.25 ml/kg/min; nAmis: 53.48 ± 6.06 ml/kg/min, p ＜ .05) and significantly higher post-exercise Lac (Lacpost) (Amis: 14.23 ± 3.29 mmol/L; nAmis: 10.66 ± 1.10 mmol/L, p ＜ .00) in maximal exercise test. There was no significant difference between 2 groups in aerobic endurance test, but Amis had significantly lower VO2 (Amis: 46.66 ± 5.16 ml/kg/min; nAmis: 51.82 ± 4.04 ml/kg/min, p ＜ .05) and Lacpost (Amis: 9.32 ± 1.94 mmol/L; nAmis: 11.11 ± 1.62 mmol/L, p ＜ .05) in anaerobic endurance test. Amis had poor maximal aerobic capacity and better lactic buffering capacity than nAmis baseball players in maximal exercise test. The lower VO2 of Amis in anaerobic endurance test is probably because anaerobic pathway was involved earlier and also there was better lactic acid neutralizing capacity. Amis group have significantly lower Lacpost than nAmis in the same relative intensity anaerobic endurance test. It shows that Amis may have superior performance than nAmis in the anaerobic endurance exercise which requires lactic buffering capacity.