The purpose of this study was to investigate the influences of alternately wearing soft and hard soled shoe on the lower extremity kinematics. Thirteen males were includes in this study to run on a motor driven treadmill with soft (shore 45C) and hard (shore 70C) soled shoes alternately. The results showed that the runners performed smaller heel impact velocity and greater knee and ankle flexion velocity after landing with the hard soled shoe. Over-tuning of knee flexion velocity after landing only occurred under the combination of wearing hard-soft-hard soles alternately. There were no any significant differences in stride frequency and length for wearing different hardness shoe. The results indicated that the runners adopted the landing strategy of smaller heel impact velocity, which leaded to a less impact energy, to reduce the impact forces as striking. The greater knee and ankle flexion velocity after landing seemed to keep the support leg stability at the stance phase. In the future, the over-tuning of knee flexion velocity after landing under alternately wearing hard-soft-hard soles and the support leg movements at the stance phase during treadmill running should be noticed.