The main purpose of this study was to understand sexaul knowledge of the psychiatric rehabilitation institution staff members. We developed a questionaire about the sexual knowledge and used it to assess sexaul knowledge of the psychiatric rehabilitation institution staff members. 193 participants were invited to fill in the questionnaire in Kaohsiung and Pintung city. Several important results were presented. First, we found the psychiatric rehabilitation institution staff members used to get sexual knowledge from the magzines and books. Second, there was a significant difference in sexual knowledge among staff members of age, education, profession and religion. According the above conclusions, the researchers lists some suggestions to the psychiatric rehabilitation institution, training institutions, staff members and other researchers.