The purpose of this study was to diagnose athletic ability using application of segmented speed analysis, the maximum speed and movement after the accumulation of lactic acid to determine 200-meter and 400-meter of athletes' ability, and give suggestions for their training program respectively. Method: Twelve male senior track athletes (six 200-meter and six 400-meter runners) participated in this study. Each 50 meters an infra-red sensor recorded the speed of the athlete. Blood lactic acid was measured after the 200-meter or 400-meter running. Results: Maximum speed was reached at 100 meters for both 200-meter and 400-meter tests, and slow down the speed too fast affected the results. In addition, after the end of the test, the maximum lactic acid occur inconsistent. Conclusion: the maximum speed of 200 and 400 meters were both reach at 50-100 m that was too early and then immediately decline that may affect with anaerobic endurance ability insufficient. Bad anaerobic glycolysis will affect the value of the maximum lactic acid and appear too early. Suggestions were to increase intermittent training and speed training to improve athlete's ability.