Shoulder pain or pathology was a common injury in overhead athletes. Overhead sports included unilateral or bilateral arms used, like baseball, tennis, or swim. Although these sports have different characteristics, they show similar overhead movement patterns. However, few studies have been conducted with athletes who share this similar overhead movement but differ in unilateral or bilateral arms used, such as swimmers, baseball or tennis players. Therefore, the purpose of this study was to measure and compare the shoulder range of motion (ROM) in healthy swimmers, tennis and baseball players. Twenty-seven overhead athletes participated in this study (7 swimmers, 11 tennis players, and 9 baseball players). The shoulder flexion, hyperextension, internal or external rotation were measured by using goniometer for both arms. One-way ANOVA and Turkey post hoc comparisons were used to analyze the difference in dominant and non-dominant shoulder amongst swimmers, tennis, and baseball players. In dominant and non-dominant shoulder, no significantly difference was found in shoulder flexion and hyperextension amongst 3 groups. The internal rotation of dominant shoulder was significantly greater in swimmers than tennis and baseball players. The external rotation of dominant shoulder was showed significant smaller in swimmers than tennis and baseball players. In non-dominant shoulder, the internal rotation ROM was showed significantly smaller in tennis players than swimmers and baseball players. There was no significantly difference in external rotation ROM of non-dominant shoulder amongst three overhead sports.