Throw-in is a method to restart a defected ball in a soccer match of eleven players. Apart from the technical skill and strength of the player, the release angle of the throw is also a factor affecting release distance. This research analyzes two commonly used ways of throw-in, investigating the variation of two different ways of throw-in (run-up throw-in and stride throw-in) with their release angles. By using a CASIO EX-F1 high speed video camera (300Hz) to collect kinematics data of twenty two subjects (average year of experience of 4.28±4.14 years, average height of 175.04±6.24 cm) using two different ways of throw-in. The release angles are analyzed by using Silicon coach Pro. The angles and distances are calculated by using Office Excel, and then analyzed through scatter plot. The result shows the best release angles are in the range of 20~40 degrees. According to statistics, run-up throw-in gives an average distance longer than stride throw-in. By hypothetical assumption, run-up throw-in has higher acceleration, letting run-up throw-in gives a longer distance.