Background: When faced with unfamiliar people and environment during hospitalization, preschool children tend to experience nursing issue, such as separation anxiety. As a result, the pain caused by invasive treatments, such as intravenous injection, will be enlarged. During intravenous injection, nursing personnel usually have to isolate family members from treatment room and use more manpower to fix sick children on treatment beds. Purposes: The main purpose of this project is to reduce preschool children's behavioral responses to intravenous injection process. Methods: This study used structured questionnaire and interview to collect the behavioral responses to intravenous injection process of 73 sick children: a prior bad experience about injection; lack of injection skill training; dull ceiling and walls. Proposed solutions: alternative injection position, re-development of pediatric intravenous injection procedures, development of adequate aids, installation of TV on ceiling, and renovation of walls. Results: After the implementation of this project, severe behavioral responses were reduced by 33.9%, reaching the target value. It is hoped that this project can be provided as reference for medical and nursing units to administer intravenous injection to sick children. Conclusions and Application: Many children tend to display behaviors of resistance when receiving intravenous injection during hospitalization. Nursing personnel found that children were unable to cooperate with them and family members usually displayed many behaviors of medical interventions. After the implementation of the project, the use of aids fixtures and distraction could effective reduce children's behavioral responses.