Purpose: The purpose of the study was to investigate the effect of backpack carrying load on walking gait parameters and inspected if it was possible to reduce injuries by increasing the thickness of the insole. Method: This study recruited 14 college participants (height :161.5±6.4cm weight: 52.2±8.4 kg), all of the participants have exercise habits (50 more hours in 1 year). All of them did not have any injuries related to neural muscular, bones, tendons and ligament as well as cardiovascular disease within six months. This study used AMTI force plate and Cortex high-speed motion capture system to collect data. The trial included the participants walking under two conditions-without load and added with 10% of self-body weight. And the insoles were then inserted to observe whether it can scale down the lower limb loading rate or not. By using two-way ANOVA to analyzed and compared the effect of walking in different load and insole thickness (α=0.05). Result: The main power, braking force, impact force, first main power and maximum force in walking will increase with the load added. The minimum hip angle would decline on the other hand. Conclusion: The results could be put into practice to improve the overload issue on backpack by starting with the materials' elastic coefficients.