The study aims at analyzing the meaning of the symbol which is about the pattern of majolica tiles by Semiotics, and applying the culture symbol of majolica tiles' pattern to poster design. This study summarizes the culture symbol of majolica tiles' pattern and clarifies the relation between signifier and signified of majolica tiles. In addition, use the combinations of symbols which is composed of Paradigms and Syntagms to be the basement patterns in the following poster design. As a consequence, the result of this study is divided into three parts which are "The Symbolization of Majolica Design", "The Visualization of Majolica Symbol" and "The design modules of Majolica Symbol poster". In the first section, divide the outside symbols of signifier into seven categories which are "The Plant Symbol", "The Animal Symbol", "The Landscape Symbol", "The Historical Figure Symbol", "The Character Symbol", "The Geometric Symbol", and "The Implement Symbol". Secondly, divide the inside symbols of signifier into three categories which are "The Natural Signification", "The Animal Signification" and "The Humanity Signification". Next, establish the form of Traditional Taiwanese Majolica Tiles symbol system by the field research that collected and analyzed the symbol of majolica tiles samples from Wufeng Lin Family. The theme of poster design is categorized into five parts which are according to the meaning of historical decorations in Wufeng Lin Family. The five themes are as follow, "Great Fortune", "Socio-economic Status", "Family", "Morality", and "Living Standards". Lastly, this study establishes the standardization of design module through design flow diagram and applies the analysis of Semiotics to the poster design.