Objectives. Mini-CEX is a useful tool for assessing clinical competences including knowledge, skills and attitude. However, lack of studies used the mini-CEX to evaluate the clinical competence in the interns of physical therapy. The purpose of this study was using mini-CEX to assess the learning outcomes of interns in physical therapy. Methods. Secondary data analysis was used to conduct this study. Data were collected from the department of rehabilitation in a medical center from July 2018 to April 2020. Four professional fields which contained orthopedic, neurology, pediatric and cardiopulmonary were analyzed in this study. Data analysis used wilcoxon signed-rank test to compare the difference between midterm and final stage of practical training. The significant level was set at p<0.05. Results. Total of 47 interns were included in this study. The scores of midterm and final stage of practical training were 3 and 4 respectively which represented that most of the interns could meet the satisfactory criterion after training. The scores of final stage improved significantly in the orthopedic, neurology and cardiopulmonary. However, in the pediatric, significant difference only showed on physical examination and counseling skills. Conclusion. Mini-CEX is a useful assessment tool for physical therapy education that can represent the learning outcomes in the practical training. Teachers in the clinics and schools can understand the students' clinical performance during internship and take as a reference for improving the clinical education.