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題名:豬肝蛋白質之水解研究
書刊名:大葉學報
作者:吳孟娟凃瑞澤吳淑姿
作者(外文):Wu, Meng-chuanToo, Jui-rzeWu, Shwu-tzy
出版日期:1998
卷期:7:1
頁次:頁143-152
主題關鍵詞:肝臟蛋白質酵素修飾水解度LiverProteinEnzymatic modificationDegree of hydrolysis
原始連結:連回原系統網址new window
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  • 被引用次數被引用次數:期刊(1) 博士論文(0) 專書(0) 專書論文(0)
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  • 點閱點閱:46
     利用五種商業用蛋白�t將豬肝蛋白質水解,實驗結果顯示,水解度與水解時間及酵素液濃度成正相關,反應經二小時後,水解速率趨於平緩,五種蛋白�t對豬肝蛋白質的水解效果高低順序分別為:Alcalase > Trypsin > Papain > Pepsin > Asp.oryzae-protease。成品之成分分析結果,水解物的蛋白質含量均不低,上澄液經噴霧乾燥後,其成品的蛋白質含量為56.62∼65.61%,未溶物成品蛋白質含量為38.20∼59.50%,而且水解後必需胺基酸含量亦未減少,經酵素處理後成品的灰分含量皆很高。
     Five commercialized proteases are used to hydrolyze porcine liver. Experiments are performed using various enzyme/protein ratios. Results show that the higher the enzyme/protein ratio, the higher the degree of hydrolysis. In general, the rate of hydrolysis reaction becomes sluggish after 2 hours. Experimental results show that the digestibility of these proteases are in the descending order of Alcalase, Trypsin, Papain, Pepsin, and Asp. oryzae-protease. Hydrolysates are then analyzed to examine the contents of protein, lipid, amino acids, carbohydrates, ash and water. The sprayed-dried supernatants of products contain 56.62∼65.61% protein, while the precipitates of hydrolysates contain 38.20∼59.50% protein. The amounts of essential amino acids do not change during hydrolysis. However, the ash contents of hydrolyzed products increase, because of the neutralization of pH with acid or alkaline. Therefore, further desalting process may be required.
期刊論文
1.廖銘清(1995)。台灣地區養豬頭數調查及其變動分析。台灣農業,31,91-97。  延伸查詢new window
2.Baek, H. H.、Cadwallader, K. R.(1995)。Enzymatic hydrolysis of crayfish processing by-products。Journal Food Science,60(5),929-935。  new window
3.Ledward, D. A.、Lawrie, R. A.(1984)。Recovery and utilization of by-product proteins of the meat industry。Journal of Chemical Technology and Biotechnology,34(3),223-228。  new window
4.Whitaker, J. R.(1963)。Determination of molecular weights of proteins by gel filtration on Sephadex。Analytical Chemistry,35(12),1950-1953。  new window
5.Surowka, K.、Fik, M.(1994)。Studies on the recovery of proteinaceous substances from chicken heads II: Application of pepsin to the production of protein hydrolysate。Journal of the Science of Food and Agriculture,65,289-296。  new window
6.戴東發(1989)。十年來猪肉藥物殘留檢驗工作之探討。農牧旬刊,19-32。  延伸查詢new window
學位論文
1.邱弘儀(1995)。屠宰場副產物肝、肺臟回收處理與功能特性探討(碩士論文)。大葉大學。  延伸查詢new window
圖書
1.張為憲(1996)。食品化學。台北:華香園出版社。  延伸查詢new window
2.張文重(1978)。蛋白質分解酵素。台北:國立編譯館。  延伸查詢new window
3.高明德(1986)。營養學概論。新竹:黎明書店。  延伸查詢new window
4.Adler-Nissen, J.(1986)。Enzymic Hydrolysis of Food Proteins。London, England:Elsevier Science。  new window
5.Tecator(1987)。Instruction Manual: Digestion System 6/12 and Kjeltec System 1026 Distilling Unit。Sweden:Tecator Co.。  new window
6.Tecator(1983)。Instruction Manual: Soxtec System HP6。Sweden:Tecator Co.。  new window
7.Regenstein, J. M.、Regenstein, C. E.(1984)。Food Protein Chemistry--An Introduction for Food Scientists。New York:Academic Press。  new window
8.AACC(1976)。Approved methods of the American Association of Cereal Chemists。St. Paul, Minnesota:Association of Cereal Chemists。  new window
 
 
 
 
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