Twenty-six Chinese female soccer players (forward 7、midfield 7、back 9 and goalkeeper 3) with age 21.0 ± 2.1 years (mean ± SD),height 162.2 ± 5.2 cm, weight 55.3 ± 5.5 kg, were accepted the run tests of 30-m、 60-m、 100-m、 200- m、400-m、800-m and 12 minutes run-walk before the 4 months of Asian games. Used one-way ANOVA to analyze above test dates that by coaches offered. Back (164.3 cm) was after goalkeeper (169.0 cm)that was the second at height among different positional players, and were better (p<.05)than midfield (158.0 cm) either. In weight, goalkeeper was better (p<.05) than the other positional players and back was better (p<.05) than midfield, also. Never found significant differences in run tests, excepted back 200-m (29.6 sec.) better (p<.05)than forward (32.2 sec.). We concluded that the defender had tall build, forward's lactate system was significant worse than back's and that could be effects in run and attack at last 15 minutes of first and second half in Chinese female soccer players.