本研究以裱敷 1mg 1-(2-pyridyl) piperazine (1,2-PP) 的玻璃纖維濾紙進行 二異氰酸甲苯（ Toluene dissocyanate, TDI ）及二異氰酸二苯甲烷（ Methylene bisphenyl isocyanate, MDI ）之採樣， 以 acetonitrile/dimethyl sulfoxide （ 90:10 ）脫附，再以 HPLC/ 螢光偵測器進行分析。 研究結果顯示，本分析方法的檢量線線性範圍 在 2,4-TDI 及 2,6-TDI 為 28 ng/mL 至 557 ng/mL； MDI 為 86.8 ng/mL 至 1736 ng/mL 。 若以 15 公升的空氣捕集量，換算為原來空氣中 2,4-TDI 及 2,6-TDI 濃度則從 3.7 μ g/m �藻� 73 μ g/m �纂FMDI 濃度為 11.6 μ g/m �藻� 231.5 μ g/m �纂A均涵蓋我國的 法定標準（ 2,4-TDI 最高容許濃度為 35 μ g/m �纂AMDI 最高容許濃度為 200 μ g/m �� ）。 本方法之分析變異係數在 2,4-TDI、 2,6-TDI 及 MDI 分別為 2.3%、 3.1% 及 1.9% (N=6)； 偵測極限分別為 1ng/mL、3ng/mL 及 14ng/mL。 三者的脫附效率分別為 108.4%、 104.3% 及 106.4%，捕集效率在 2,4-TDI 及 2,6-TDI 分別為 89.35% 及 94.33%。 無論是 裱敷濾紙或樣品濾紙， 以室溫遮光或冰箱冷藏（ 4 ℃）保存，均可穩定至少 28 天且偏差 小於 10%。本研究方法適合作為我國作業現場 TDI 及 MDI 短時間暴露濃度標準之測定方法 ，並已克服過去現場採樣分析上之困難。

　　　　　A sensitive high-performance liquid chromatography (HPLC)-fluorescence detection method for the determination of toluene diisocyanate (TDI) and methylene bisphenyl isocyanate (MDI) is described. Glass fiber filters coatd with 1mg of 1-(2-pyridyl) piperazine (1,2-PP) are employed to collect airborne TDI and MDI. After the air sampling, the filter is extracted with 90/10(v/v) acetonitrile/ dimethyl sulfoxide.Result shows that the linear dynamic ranges are from 28 to 557 ng/mL for 2,4-TDI and 2,6-TDI and from 86.8 to 1736 ng/mL for MDI. The corresponding air concentrations are from 3.7 to 73 μ g/m �� for 2,4-TDI and 2,6-TDI and from 11.6 to 231.5 μ g/m �� for MDI based on a 15 liters air sample. Result also indicates that the analysis coefficients of variation (N=6) are 2.3%, 3.1% and 1.9%, and the extraction efficiencies are 108.4%, 104.3%, 106.4%, respectively, for 2,4-TDI, 2, 6-TDI, and MDI. The detection limits for the three compounds are 1ng/mL, 3ng/mL and 14ng/mL, respectively. The sampling efficiencies of 2,4-TDI and 2,6-TDI are 89.35% and 94.33%. The coated filters are found stable for at least 28 days after collection of samples, with error less than 10%, irrespective to sample storage in room temperature or in a refrigerator. This method is recommended to be use for the analyses of short-term exposure level of TDI and MDI in the field.