Objectives: Due to the interest in the effectiveness and appropriateness of hormone replacement therapy (HRT) for menopausal women in Taiwan, we investigated the hormone level of follicle stimulating hormone (FSH) and estradiol (E2) for their value and affecting variables. Method: 753 women prior, during, and post to their menopausal periods who checked in at a clinic for routine wellness exams were invited to participate in this study. Measurements included serum level of FSH and E2 and self-reported que stionnaires on physical and mental wellness as well as those health behaviors. Results: There were no significant association between the subjects' physical and mental discomfort and their FSH and E2 levels. The correlation between FSH and sexual discomfort was significant, but the correlation coefficients were small. The average FSH level for post-menopause women was greater than 40 m IU/ml. The range of E2 level were 41-85 pg/ml for subjects without or with medication, two times greater than the standard 20 pg/ml, level used to determine need for HRT supplementation. A multivariable regression with the inclusion of age, menopausal status, BMI, use of hormone, and smoking status in the model explained approximately 50% of FSH level for women. Among these variables, age alone explained about 39% of the FSH level, and 22% of the E2 level. Relatively, the menopausal status contribution was minor. Conclusions: hormone level does not explain the menopausal discomfort for women. The E2 level may not be an approp riate indicator for hormonal therapy prescription. Furthermore, how to find objective indicators and attention on health behaviors affecting the level of FSH should be studied in the future too.