Poverty has been one of main topics worldwide. The poverty-stricken population tends to gather in some particular space with spatial characteristics, especially in the mountainous area. Guided by the research framework of the Sciences of Human Settlements, this paper builds a theoretical model showing the connection between spatial factors and poverty, which contains the factors of five aspects: natural resource, human, economy and society, city and town, and infrastructure. Using data from 75 counties of Guizhou Province, the research finds that the influence is positive by using partial least squares regression. The result shows that there is a wide correlation between the many spatial factors and the generation of poverty, and that there is intercoupling between the peopleland contradiction, the weak economic integration capacity, and the lagging spatial supporting capacity, which is the main spatial mechanism for the generation of poverty. In the end, the paper puts forward corresponding suggestions for spatial planning and construction.