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題名:體外胚囊發育進程源自僅用乙醇曝置達成孤雌激活的體外成熟豬卵母細胞
書刊名:弘光學報
作者:林育安蔡宏斌陳銘正
作者(外文):Lin, Yu-anTsai, Hong-bingChen, Ming-cheng
出版日期:2011
卷期:65
頁次:頁154-166
主題關鍵詞:豬卵母細胞體外成熟孤雌激活Porcine oocyteIn vitro maturationParthenogenetic activation
原始連結:連回原系統網址new window
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本試驗之目的,乃研究不同濃度之乙醇(0、3、5、7與9%)與選定濃度之乙醇(7%)以不同時間(0、1、3、5與7分鐘)測試對體外成熟豬卵母細胞激活之影響。豬卵母細胞經體外成熟培養48小時後,分別置於不同濃度之乙醇各5分鐘與置於7%乙醇中各0、1、3、5 與7分鐘,進行豬卵母細胞之激活刺激,隨後並培於NCSU-23培養液養分別培養16小時與144小時觀察卵母細胞激活與胚發育情形。試驗結果呈現,7%乙醇分別以0、1、3、5 與7分鐘對豬卵母細胞激活之卵分裂率分別為7.6、12.0、44.9、63.0與52.3%,以5分鐘組之胚發育比率最高(P < 0.05),胚囊發育比率也以5分鐘組最高(P < 0.05),顯示體外成熟之豬卵母細胞可以僅用7%乙醇施以激活5分鐘的最佳處理條件,獲致顯著的體外胚囊發育進展。
The objective of this study was to investigate the activation effects at various concentrations of ethanol (0, 3, 5, 7 and 9%) for regular exposure duration (5 minutes) and as well at specified concentration of ethanol (7%) for various exposure duration (0, 1, 3, 5 and 7 minutes) on the in vitro matured porcine oocytes. Porcine oocytes separated from same preparation batch were exposed for 5 minutes at various ethanol concentrations and as well were exposed for 0, 1, 3, 5 and 7 minutes at 7% ethanol after maturation in culture for 48 hours. Subsequently, both activation and embryo development of the activated oocytes were respectively observed for 16 h and for 144 h in NCSU-23 embryo culture medium. The results indicated that the cleavage rates upon oocytes activation for 0、1、3、5 and 7 minutes group by 7% ethanol were 7.6、12.0、44.9、63.0 and 52.3%, respectively. Moreover, the observed highest cleavage rates upon 5 minutes exposure among other groups (P < 0.05) has also shown higher blastocyst rates among other groups (P < 0.05). The present study indicated that the 7% ethanol only exposure for 5 minutes may serve optimal activation on in vitro matured porcine oocytes with significant in vitro blastocyst progression.
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