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題名:不同運動項目選手血清對人類肝癌細胞株HepG2生長及抗氧化之影響
書刊名:全民休閒發展學刊
作者:何應志蔣明雄 引用關係朱碧梧 引用關係陳福安許美智 引用關係
出版日期:2011
頁次:頁83-99
主題關鍵詞:硫巴比妥酸反應物質乳酸脫氫酶
原始連結:連回原系統網址new window
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本研究之研究目的為比較舉重選手、長跑選手及坐式生活者之血清對肝癌細胞株H e pG2 之生長影響; 並採用硫巴比妥酸反應物質(thiobarbituric acid reactive substance; TBARS )及乳酸脫氫酶((lactate dehydrogenase;LDH) 生物分析法來評估比較舉重選手、長跑選手及坐式生活者之血清對肝細胞之抗氧化保護功能。本實驗分別取經長期運動訓練長跑選手、舉重選手、以及坐式生活者各1 2 位之血液,並使用肝癌細胞HepG2為模式細胞株, 比較不同血清對肝癌細胞HepG2 之生長影響,以及不同血清對細胞的抗氧化保護能力。結果顯示, 在不同血清對HepG2 生長之影響上, 舉重組在5%與2 0%血清濃度之細胞數明顯高於長跑組( p < . 0 5 ); 坐式組在5%與1 0%血清濃度之細胞數也明顯高於長跑組( p < . 0 5 ) ; 而舉重組與坐式組則沒有明顯差異之存在。在LDH 活性百分比之比較上, 舉重組在5%血清濃度上明顯低於長跑組, 在2 0%血清濃度時明顯低於其他兩組( p < . 0 5 ), 長跑組與坐式組則無明顯之差異。在TBARS 產生量百分比之比較上,各組間並無顯著之差異。研究結果顯示,長跑選手血清組之細胞生長數較少, 以及抗氧化保護功能較差, 血清中的抗氧化物質濃度相對偏低, 建議長跑選手應更注意抗氧化劑之補充。
期刊論文
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9.Wallin, B.、Rosengren, B.、Shertzer, H.G.、Camejo, G.(1993)。Lipoprotein oxidation and measurements of thiobarbituric acid reacting substances formation in a single microtiter plate:its use for evaluation of antioxidants。Analytical Biochemistry,208,10-15。  new window
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13.Helen, A.、Krishnakumar, K.、Vijayammal, P.L.、Augusti, K.T.(2000)。Antioxidant effect of onion oil (Allium cepa. Linn) on the damages induced by nicotine in rats as compared to alpha-tocopherol。Toxicology Letters,116(1/2),61-68。  new window
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16.Lusini, L.、Tripodi, S.A.、Rossi, R.(2001)。Altered glutathione anti-oxidant metabolism during tumor progression in human renal-cell carcinoma。International Journal of Cancer,91,55-59。  new window
17.Martensson, J.、Meister, A.(1992)。Glutathione deficiency increases hepatic ascorbic acid synthesis in adult mice。Proceedings of the National Academy of Sciences of the United States of America,89(23),11566-11568。  new window
18.Meister, A.(1991)。Glutathione deficiency produced by inhibition of its synthesis, and its reversal; applications in research and therapy。Pharmacology & therapeutics,51,155-194。  new window
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21.Roe, A.L、Snawder, J. E、Benson, R. W.、Roberts, D. W.、Casciano, D. A.(1993)。HepG2 cells: an in vitro model for P450-dependent metabolism of acetaminophen。Biochemical & Biophysical Research Communications,190,15-19。  new window
22.Strom, S.C.、Jirtle, R. L.、Jones, R. S.、Novicki, D.L.、Rosenberg, M.R.、Novotny, A.、Irons, G.、McLain, J.R.、Michalopouos, G.(1982)。Isolation, culture, and transplantation of human hepatocytes。Journal of the National Cancer Institute,68,771-778。  new window
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圖書
1.Jackson, M.J.(2000)。Exercise and oxygen radical production by muscle。Handbook of Oxidants and Antioxidant in Exercise。  new window
 
 
 
 
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