Spiritual well-being is a feeling of purpose of life and life satisfaction. Although studies mentioned there are closely relationship among spiritual well-being, symptoms disturbance, mental health and physical function, But currently understand about the spiritual well-being of older persons is very limited, and there have not been reported about spiritual well-being of hospitalized elderly. The purpose of this study is to understand the spiritual well-being of hospitalized elderly and the influence factors. Cross-sectional survey research, study subjects were hospitalized elderly patients for a medical center, from June 1 to December 31. Instruments included geriatric comprehensive assessment, and spiritual index of well-being Chinese version. Descriptive statistics, t test, ANOVA test and Pearson’s correlation were used. A total of 150 subjects, majority were poly-pharmacy (taking four or more medications) (n=101, 67.3%), mean and standard deviation of spiritual index of well-being Chinese version was 30.71 ± 10.35. Mean of Eurogol-5D-3L was 7.14 ± 2.43, EuroQol-VAS was 66.39 ± 19.74, belong to "medium" of the standard. The study found that hospitalized elderly feelings on health, quality of life, depression status, instrumental activities of daily living, had significantly correlated with their spiritual well-being. When self-perception of health, quality of life was good, no depression, and instrumental activities of daily living was good, then the state of spiritual well-being was good. Also there are significant difference between poly-pharmacy, sleep disturbance and spiritual well-being, taking more than four kind of medication a day or people who had sleep problems, there spiritual well-being was poor conditions. The results of this study can help healthcare providers understand the influence factors of hospitalized elderly spiritual well-being, and as a reference to the relevant care plan. The research results also can be the basis of designed nursing interventions to promote spiritual well-being and quality of life of elderly people, and promote to achieve the holistic health care goals.