Most of the time, experimental animation displays artists’ subjective consciousness and their interpretation to the social environment. It also applies contemporary cultural and aesthetic thinking to yield more open and varied creative expression. Mixed medium is often used in experimental animation to enable free and complex visual aesthetics which will give a unique visual perception. This paper researches two aspects-concept for original artworks and experiments with the medium. In the first research, the concept for original artworks that animation presents and brings about under the influence of Dadaism, Surrealism and Digital Arts are explored. In the second research, covering form non-digital medium experiments to digital mixed medium, works of animation and discussions are used to consider the experimental nature of animation. This paper shows nature of experimental animation and its subsequent mutual application between concept and medium so that animations can become more profound and creative.