The comfort of a classroom will influence the student’s learning. Most of Taiwanese school administers provide air conditioners to improve the comfortable environment to improve students’ learning. However, the feelings of students in this circumstance were less discussed. The research used ASM to measure indoor environmental physical factors. It took students’ personal data and held subjective comfort questionnaires as well. The physical experiment had conducted in two kinds of status, air-conditioner and non air-conditioner. We selects T1-303、T1-305、T2-109 and T2-114 classrooms in CYUT to be the test targets. The results show that the claimed comfortable temperatures are 25.8℃ (43%RH) with air condition and 27.5℃ (42%RH) without air condition, respectively.