According to the literature survey, there are still very few research focusing on the online live instruction in e-learning. By integrating IP-Based video conferencing system and full screen capture with live streaming broadcast system into an LMS, instructors can provide online live instructions. In this kind of environment, teachers and students can do two-way interaction just like in conventional classroom. This study explore the process of online live instructions and provided solutions for the encountered problems. SOP (Standardized Operation Procedures) are developed on how to conduct course’s online lecturing and group online live discussion & oral report presentations. Suggestions about online live instruction for teachers, teaching assistants and students are also included.