The study was to understand the physiological changes of Qi-Gong learners in their Qi-Gong state, concerning their EMG value, skin temperature, and periphery blood volume. Twenty-four subjects were divided into a Qi-Gong group and a control group. The main device of the experiment was "Pro Comp Multi-functional Physiological Feedback Monitoring System" which allowed simultaneous monitor on the EMG value, the skin temperature, and the periphery blood volume of the subjects. In the beginning of the experiment, both groups undertook the first test. After the Qi-Gong group practicing Qi-Gong for fourteen weeks, both groups undertook the second test to check up the EMG value, the skin temperature, and the periphery blood volume of the Qi-Gong group in their Qi-Gong state, while the control group in their relaxed state. After collecting the data as the results of the experiment, the EMG value was evaluated by independent sample t test for the agreement between the first test and second one. The skin temperature and the periphery blood volume of the two groups in the first test were evaluated by independent sample t test for the agreement between the two groups. The skin temperature and the periphery blood volume of the two groups in the second test were evaluated by independent sample t test for the difference between the two groups. The findings were as follow: Firstly, as far as the EMG value of the Qi-Gong group and the control group was concerned, there was no significant difference between the first test and the second one, which showed that the two groups reach an agreement between the first test and the second one. Secondly, as far as the skin temperature and the periphery blood volume were concerned, there was no significant difference between the Qi-Gong group and the control group in the first test, which showed that there was an agreement between the two groups. Besides, as far as the skin temperature and periphery blood volume were concerned, there was a significant difference between the Qi-Gong group and the control group, which shows that after a short-term Qi-Gong training, the Qi-Gong state had a significant effect on human physiological response.