This study analyzed the cadets physical fitness performance of National Defense University, Participants of this research are 715 male cadets of Chung Cheng Institute of Technology, National Defense management College and Fu Hsing Kang College of National Defense University. The average age of the Participants is 20.42. During physical education to inspect the cadets’ physical fitness of 1-minute-sit-up, 1-minute-pull-up and 3000 meters run. Results of the experiment are analyzed by SPSS for Windows, All analyses of this research are based on significant level (α=.05). Through data analysis, the results are as follows: (1) The different military academy hold significant difference in their physical fitness performance in sit-up, pull-up and 3000 meters run. (2) The different years cadets hold significant difference in term of their physical fitness performance in 1-minute-sit-up, 1-minute-pull-up and 3000 meters run. (3) The age, stature, weight and body mass index with 1-minute-sit-up, 1-minute-pull-up and 3000 meters run have conspicuous relation.