Objectives: Patients with obesity face increased risks in operations. Moreover, during the postoperative patient handling procedure, potential risks that may cause patient injuries and caregiver muscle or bone injuries exist. Hence, the present study used the root cause analysis squared (RCA²) to identify the root causes for the ineffective postoperative patient handling practices and establish a standardized set of postoperative patient handling procedures to reduce the occurrence of adverse events during the patient handling procedures. Methods: An RCA² team was formed in June, 2015, to identify the root causes for patient slippage during the postoperative handling period, and also to establish standardized procedures for the handling and determine the clinical effectiveness of such interventions. Results: The project lasted from June, 2015 to August, 2016. After the introduction of the intervention, among the patient handling procedures, which had an executive rate of 96.1% from May to August, 2016, no instances of handling-related adverse events occurred. Conclusions: The RCA² is an effective tool to locate possible causes of problems in an effort to improve service quality, establish and implement safe patient handling procedures, enhance patients' safety in the postoperative period, and prevent adverse events.