The purpose of this study is to explore the impact of Naval staff work rotation on job satisfaction and to use the perceived work rotation as a mediator variable. In this study, a convenient sampling method was used to issue 300 questionnaires. 235 valid samples were recovered, and the effective questionnaire recovery rate was 78.3%. The results of this study are: (1)"Working Rotation - Meaning Perception", "Working Rotation - Career Development Cognition" and "Working Rotation - Rotational Willingness" have a positive impact on "perceived work rotation". (2)"Perceived work rotation efficiency" has a positive impact on "job satisfaction - job performance" and "job satisfaction - job prospects". (3)"Working Rotation - Meaning Perception", "Working Rotation - Career Development Cognition" and "Working Rotation - Rotational Willingness" vs. "Work Satisfaction - Job Performance" and "Work Satisfaction - The work outlook" has a positive impact. (4) "Perceived work rotation effect" has a partial mediation effect on "work rotation - meaning perception" and "job satisfaction - job play"; "Perceived work rotation efficiency" has a complete mediation effect on "work rotation - career development awareness" and "job satisfaction - job play"; "perceive work rotation effect" versus "work rotation - rotation willingness" and "Work Satisfaction - Job Performance" has a full intermediary effect. (5) "Perceived work rotation effect" has a partial mediation effect on "work rotation - meaning perception" and "job satisfaction - work prospect"; "Perceived work rotation efficiency" has a partial mediation effect on "work rotation - rotation willingness" and "job satisfaction - work prospect"; "Perceived work rotation efficiency" has a complete mediating effect on "work rotation - career development cognition" and "job satisfaction - work prospect". (6) "Service Years" has significant differences in "job satisfaction - job performance" and "job satisfaction - job prospects". The nature of work has significant differences in "job satisfaction - job play".