Objectives: After a diagnosis of breast cancer, patients would be more likely to experience preoperative anxiety due to the fact of having cancer and the pressure of operations. In this study, the effect of multimedia education was examined. Methods: This study adopted a quasi-experimental design, and data collection started from January 1, 2017 to March 31, 2018. The subjects who underwent a mastectomy for breast cancer were 60 patients. There were 30 patients who accepted traditional routine education in the control group and 30 patients who accepted traditional routine education combined with multimedia in the experimental group respectively. For the patients who were initially diagnosed with breast cancer and needed a mastectomy, the difference of anxiety scale between control group and experimental group was compared. Results: This study found no significant difference in total questions, but significant differences in five individual questions between the control group and experimental group. This result showed that traditional routine education combined with multimedia would have partial effects of anxiety reduction for pre-operative breast cancer patients. Conclusions: The conclusion was that traditional routine education combined with multimedia would have the effects of emotional stability for pre-operative breast cancer patients.