Fifty-five high school female students with age 16.3 ± 0.4 years (means ± SD), height 159.2 ± 4.6cm, weight 52.1 ± 8.0kg, were assigned randomly into either 4 experimental groups or a control group. Whole groups had normal physical activities durign the 5 weeks period, except the 4 experimental groups added 2 days per week, three sets per day; three motions with three times per set static stretching training. According to different holding time to set apart the groups to 5, 10, 30 and 40 second after stretching, and those groups had the same 30 second to take a rest, but the control group did not receive regular training. Sit and reach test was evaluated between before, middle (three weeks later) and after 5 weeks period, then we used one-way ANOVA, it appeared the middle test better than the before and the after also better than the middle one. It represented that more static stretching exercises in 4 experiment groups got the more effects of flexibility. Never found that longer holding time after stretching could improve more flexibility. The control group was no significant changes (p>.05) in three tests. We concluded that static stretching exercise was really helpful to subject's flexibility of lower back and hamstrings, and the effects of 5 weeks better than 3 weeks. But no matter short or long in holding time after stretching that maybe not the one of important factors in improvable flexibility.