Purpose: This study analyzed energy expenditure on sitting with sedentariness between the general chair and the balance ball within an hour. Method: Before the experiment, three males and three females needed to fill in consent and physiological survey, and completely realized the experimental procedure. In the procedure, the participants were fasted for four hours and sat still for fifteen minutes, and then sat with sedentariness for one hour. Specially mentioned, in order to reduce the interference of physiology by the chairs, one participant had to sit these two kinds of chairs on two days. In this study, samples of energy expenditure, oxygen consumption, heart rate, ventilation volume, and respiratory exchange ratio were analyzed by t-test separately. Results: The results show that heart rates, ventilation volumes, and respiratory exchange ratios between general chair and balance ball were quite the same. Energy expenditure and oxygen consumption of sitting on the balance ball were higher than the general chair. Conclusion: The duration of experiment was designed for one hour. In the duration, energy expenditure of sitting on the balance ball was higher than the general chair.