Kinmen Agreement is the first regional agreement on suspects’ pursuance with nature of criminal judicial assistance. Cross-Strait Agreement on Cooperation on Combating Crime and Mutual Judicial Assistance signed on April 26, 2009 settled up a new mutual judicial assistance pattern and system under the frame of “One Country, Two Systems”. This article comprehensively explicates nature and specialties of Cross-Strait Agreement on Cooperation on Combating Crime and Mutual Judicial Assistance through discussing derivation, character, objective evaluation and the connection between the two agreements and concludes that Cross-Strait Agreement on Cooperation on Combating Crime and Mutual Judicial Assistance will deeply influence not only the formation of our regional judicial assistance system, but also theory and practice of our regional mutual assistance.