In order to explore the relationship between work-family conflict and preschool teachers’ turnover intention, 992 preschool teachers were recruited to complete the Work-Family Conflict Scale, Professional Identity Scale, Job Burnout Scale and Turnover Intention Scale. The results showed that: 1) Work-family conflict, professional identity, job burnout and preschool teachers’ turnover intention were significantly related with each other. 2) Work-family conflict had a positive effect on preschool teachers’ turnover intention. 3) Work-family conflict had a positive effect on preschool teachers’ turnover intention through the meditating role of professional identity and job burnout. Professional identity and job burnout play a sequential mediating role in the relationship between work-family conflict and preschool teachers’ turnover intention.