The purpose of this study was to analyze the influencing factors on airline passengers’ choice behavior of on-line ticketing. It focused on the passengers’ ticketing behavior in the route of Taipei-to-Tainan and applied the binary logit model to construct an on-line ticketing demand model. The findings included: (1) the usage of on-line ticketing is still under 15% for the time being, because most respondents were not confidence with the transaction security of Internet as well as they preferred more flexible tour schedules; (2) the significant influencing variables on the on-line ticketing demand were ticket price, Internet transaction security, passengers’ trip characteristics as well as passengers’ experiences of using on-line ticketing; (3) competitive pricing, transaction security upgrading and encourage passengers to use on-line ticketing were the effective ways of motivating the on-line ticketing demand; (4) airline and ticketing website should re-exam the marketing strategies of on-line ticketing in force to fit passengers’ needs of flexible schedules and provide on-line ticketing passengers more consummate services at the airline check-in counters.