This study aimed to explore the ball service of world's outstanding female tennis players, with the focus on the differences of service spin, fall point of ball and service type. The female tennis players in the finals of the 2009 WTA Tour, who won the competitions in L.A., Cincinnati, Toronto, and Stanford, were taken as the research objects, with 86 rounds of competitions as the research scope, and the record form for ball service as the source of data for the research method. The collected data were analyzed statistically via percentage comparison and Chi-square test. The conclusions elicited from analysis and discussion, are as follows: 1 .As for the service spin among the world's outstanding female tennis players, the flat stroke is the most common (36.86%), followed by the topspin (36.588%); the ball is served mostly toward the receiver’s body or the center of the tennis court (38.28%), followed by the direction toward the alley (36.16%); in the service types, the sidespin service towards the alley is the majority of cases (19.6%), followed by topspin towards the receiver’s body or the center of the court (17.94%). 2. Several variances (the service spin, fall point of ball, and service type) reach significant differences.