The purpose of this paper is to study the stride of good runner in 24hrs Ultra Marathon Race. Take the champion–Japanese competitor Ryoichi Sekiya and the third place-Taiwanese competitor Sheng Ming Wu in 2007 Soochow International 24hrs Ultra Marathon Race as subjects. Research method is recording the runners' performance within 24hrs then split it into six sections. Compare every section with each other and analyze the result. The following are the consequence: (1) the male runners should be endurance and perseverance. In addition, the average race pace of them should be over 11 kilometer per hour. (2) Both competitors’ running stride length became smaller as the race went by. Besides, every stride spent more time than before, and the average speed also decreased. The above showed up in the fifth section (about 17 to 20 hrs). It might because the 17 hour was one o'clock at midnight, which was the sleeping time for biological clock. In addition, the decreased cheerers might be another reason for the result. (3) The length of legs might influence the stride length of running pace. The result of 2007 Soochow International 24hrs Ultra Marathon Race might influenced by the stride length of running pace more than the frequency of running stride rate (sec/step).