Purpose: The aim of the present study was to investigate the differences on elite coaches' substitute execution. Methods: Six level one male university volleyball teams were included in the present study and their semi-final games results of 2009 season were collected. Descriptive statistics and ANOVA were conducted on all of the game-related statistics. Results: Results showed that substitution frequency for service and defense section was higher than for attack and set one. When score was behind less than five points, coaches excused more substitutions than other time. When teams were leading less than five points, substitution for servicer was more welcome. When losing less than five points, more substitutions for defense and service were used. Conclusion: The purpose of substitution on service and defense was more frequent used on elite university volley coaches, especial during the tie game situation. When teams were leading less, the coach should consider to substitute players who were more excel at receive and defense, cooperated with the Libero to reverse and come from behind.