The study aimed to investigate hospital-affiliated nursing homes’ ability to apply knowledge management, and their organizational effectiveness, and also the relations between the above two items. Research methods are used cross-sectional study approach, the development of research tools development department reference from medical institutions knowledge management scale and organizational effectiveness scale, which was developed by Li-Chen Hung etc. (2007). Information recovered first to descriptive statistics, item analysis, factor analysis and reliability analysis to data processing, finally then stepwise regression model was discussed in knowledge management to organizational effectiveness. Findings demonstration, “the new knowledge acquisition, the using and the share”is helpful in promotes the organizational effectiveness, and reaches 38.3% explanation amounts of variation. Therefore, It’s necessary for induction knowledge management idea and the concrete procedure to hospital-affiliated nursing home.