This study adopts a quasi experiment method to utilize an instructional experiment via frog ecological lessons and inquire into how the lessons influence the knowledge of and the attitude toward frog ecological lessons for Grade- students. The learning process record was engaged with qualitative data. The quantity analysis results found the influence of the pre-test points was excluded via independent ANCOVA; the experiment group displayed higher points in knowledge (F=57.734, p=.000 <.05) and attitude (F=57.734,p=.000 <.05) than the control group after the instruction. This indicates the instructional experiment affected the knowledge of and the attitude toward frog ecology for students. The results of the qualitative analyses found the students and the instruction observers expressed recognition of the in-school frog ecological lecture lessons, ecological game instructional activity and the night-time field-observation instruction. This was especially the case in ecological game instructional activity and the night-time field-observation instruction. There was a lot of positive feedback from the learning sheets of the experiment group. Finally, by offering the frog curriculum design and development method, evaluation of teaching effects, and the records of teaching, this method will benefit other teachers who would like to combine environmental ecological education with formal education as teaching materials. At the same time, it also combines the teacher’s own specialty and interests into the educational curriculum in ecological research that includes additional species.