Purpose: This study compared and analyzed the differences between the offensive and defensive skills of women's single matches in Grand Slam Tournaments. Mothod: Data were collected based on the tennis players who qualified to the third round in the four Grand Slam Tournaments in the year 2013-2015. Methods: To use spss 20.0 T test and One-way ANOVA were used to compare fourteen skill variables of each single match performed on different court surfaces. Results: The results revealed that: 1. To sum up the total factors between winners and losers in 2013-2015 grand slam tournaments, The three-year results showed significant difference (p＜.05) in winning % on 1st serve PTS and winning % on 2nd serve PTS, Ace. 2. One-way ANOVA were used to compare eight skill variables of each single match performed on the different court surfaces. The results revealed in 2013 the result showed significant (p＜.05) difference in 1st Serve ％ and ace, in 2014 the result showed significant difference in winning % on 1st serve PTS, ace, fastest serves speed, Average 1st Serve Speed, Average 2nd Serve Speed, in 2015 the result showed significant difference in winning % on 1st serve PTS, winning % on 2st serve PTS, ace, fastest serves speed, Average 1st Serve Speed, Average 2nd Serve Speed. Conclusion: The result showed from the research, the key point of success or failure will be influenced from winning % on serve PTS from The Big Four Tennis Tournament , the winner player needs to possess the higher of winning ％ on 1st and 2nd serve PTS, the probability to win be enhanced, and the ace, Average 1st Serve Speed, Average 2nd Serve Speed tospeed faster of Wimbledon court will be more important.