Italy, indeed, is a country with plenty of experiences in upholding referendums. This article is to comprehend its institutional designs and practices in a long period of time. Moreover, we try to use 2016 Referendum as a case study to explore the influence of European Union in all aspects. In the end we use Causal Layered Analysis to dissect and conclude the complicated issues involved in 2016 case. We find that the litany problem is seen in governmental ineffectiveness, corruption, economic recession, and the influx of refugees. In systemic level we contend the constitutional design for Italian governments are imposing obstacles on pragmatic operations of political affairs. Also the restraints from EU jeopardized its effective functions. The world view is on how Italians see Italy and EU collaborate in political and economic issues. The last of myth we find out by blaming populism is very convenient and mess up result with origin.