Purpose: The study aimed to verify the effects of Qi Gong regimen exercise in middle-aged and elderly practitioners. Methods: There were totally seventy subjects, divided into three groups: long-term, LT (male 9 and female 10); 3 months experiment, 3-M (male 5 and female 20) and control, CTL (male 11 and female 15), with mean age of 60.56±10.23years, height of 160.60±5.26cm, weight of 62.91±7.76kg and BMI index of 24.35±2.40. The variable items of the Ryodoraku bio-energy of whole body analysis were chosen for this investigation, and evaluated the effects of pre and post during 3 months Qi Gong regimen exercise. We focused on the physiological range by using of the 24 points of mean bio-energy, upper/lower ratio to assess the health condition. The SPSS 12.0 and Excel 2000 for window version statistic analysis, one way ANOVA was conducted to identify the effects and the significance was set at α=.05. Results: They were significant difference in pre test as showed following of LT (87.40μA)>3-M (62.83μA) and also LT>CTL (65.42μA) (p<.05). There were significant difference in the changing rate (⊿%) among three groups, as showed that 3-M (25.62±48.29%)>LT (20.09±30.30%), CTL (20.70±43.88)>LT (20.09±30.30%, p<.05). It also revealed significant difference in the upper/lower ratio as that LT (1.0±0.30)>3-M (0.80±0.23)>CTL (0.78±0.19, p<.05) and demonstrated the long-term group have the better Qi and blood well balance of the Zang Fu, muscles, and bone system than the other groups. Conclusion: This research reveals that the long-term regimen exercise may affect the movement function of neuromuscular and bone's state of balance.