The purpose of this study was to investigate the effects of low- to moderate-intensity aerobic exercise training (40-50%) on functional fitness and microelectric conductivity of the 12 main meridians amongst older adults in north Taiwan. Sixty participants aged ≤ 70 years without any joint range-of-motion limitation or cognitive impairment were enrolled. After training the participants twice a week for 12 weeks, we collected data on their functional fitness and microelectric conductivity at 24 points in the main meridians. A comparison of the treatment group's results with those of a control group revealed that the former did not display notable improvement in muscle strength, muscular endurance, cardiopulmonary capacity, static and dynamic balance, or flexibility. Moreover, the microelectric conductivity values of the bilateral heart, spleen, liver, bladder, right triple energizer, and left small intestine meridians were significantly different from those of controls. We conclude that the aerobic exercise changed the microelectric conductivity of meridians associated with respiratory, cardiovascular, and urinary systems amongst older adults. Future studies could focus on increasing the duration of the exercise intervention to improve muscle strength, flexibility, static, and dynamic balance. The measurement of microelectric conductivity of the meridians to determine the effectiveness of the training program can be quantified by using a combination of physiological parameters described in the theory of traditional Chinese medicine and modern sports medicine.